The Blues skipper took to Instagram on Tuesday to confirm that he would miss the Stamford Bridge clash with the French champions.
"I'm gutted to be missing the PSG game," stated the Chelsea defender.
"It's so frustrating missing any game,but these big champions league nights at the bridge are unbelievable and it hurts as a player not to be involved
"I have thrown everything at my injury trying to get back fit but sometimes you have to let the injury settle, and thankfully I'm on the mend and hope to be back soon."
The defender has missed the club's last five games with a hamstring problem that he picked up in the 5-1 win against Newcastle on February 13.Terry had trained alone earlier in the day.
Guus Hiddink's men trail 2-1 on aggregate after the first leg at the Parc des Princes, and are also without French centre-half Kurt Zouma.
This is the third consecutive year in which Chelsea have faced PSG in the competition, with the Blues winning at the quarter-final stage in 2014 on away goals, while PSG triumphed via the same method in the last-16 a year ago.
The first leg in Paris saw the French champions inflict Chelsea's only defeat under interim boss Hiddink, although John Obi Mikel netted what could be a crucial away goal.
In pictures - Chelsea train ahead of PSG clash:
Blues chief Hiddink had already resigned himself to having to do without Terry, after the ex-England skipper was unable to take part in a training game on Monday.
“I don’t think John will make it on Wednesday. For the time being, there has not been enough development to put him on the list.
“It’s not worse – he trained and had treatment on Saturday morning, and he can run, but he cannot go into a longer stretch where you have to accelerate. There he has to be careful.
“When he is ready to do so, maybe on Tuesday, he will play in a practice match, but I don’t think he will make it. Hopefully, let’s see what can be done for next weekend.”